How to Make a Lineweaver Burk Plot in Excel: Easy WaysUpdated a month ago
Creating a Lineweaver-Burk plot in Excel allows you to linearize enzyme kinetics data for determining Km and Vmax values. Start by entering your experimental data: list substrate concentrations ([S]) in one column and reaction velocities (V) in another.
Key Highlights: Make a Lineweaver-Burk Plot in Excel
- Prepare Your Data:
Enter substrate concentration ([S]) and reaction velocity (v) in Excel. Example data might include different concentrations of substrate and their corresponding reaction rates.
- Calculate Reciprocals:
Calculate 1/[S] (inverse of substrate concentration) and 1/v (inverse of reaction velocity) in separate columns.
- Create a Scatter Plot:
Highlight the 1/[S] and 1/v data. Insert a Scatter Plot to visualize the data points.
- Add a Trendline:
Right-click on any data point, select Add Trendline, and choose Linear. Display the equation and R² value on the chart for analysis.
- Analyze the Trendline Equation:
The trendline equation will be in the form y = mx + b. Slope (m) = Km/Vmax, and y-intercept (b) = 1/Vmax. Use these values to determine Km and Vmax for enzyme analysis.
By following these steps, you can effectively create and interpret a Lineweaver-Burk plot to analyze enzyme kinetics in Excel.